Bacteriology of endometritis and in vitro antibiotic sensitivity of the isolates in Holstein Friesian and Jersey cows maintained at Research Institute for Physiology of Animal Reproduction, Bhunikey, District Kasur were carried out.Out of 100 samples, 89 contained different strains of bacteria and 11 were found bacteriologically sterile.Different species animed blue lotion topical spray of bacteria isolated from these samples were, Bacillus subtilis (08.99%), Corynebacterium pyogenes (19.
10%), Escherichia coli (29.21%), Neisseria meningitides (03.37%), Staphylococcus aureus (23.60%), Streptococcus pneumonia (03.
37%) and Streptococcus pyogenes (12.36%).The in vitro antibiotic sensitivity test indicated that the highest number of isolates (92%) were sensitive to neomycin, followed by doxycyline (89%).Clindramycin showed teal horse blanket the lowest results in terms of in vitro antibiotic sensitivity (51%).